In the present study we sought to elucidate the impact of ciprofloxacin on sperm chromatin integrity and sperm DNA damage using Aniline Blue and Acridine Orange technique, respectively. Determination of DNA integrity using a modified alkaline single cell gel electrophoresis Comet assay The following procedure adapted from Hughes et al.
The proximal centriole enters into the egg during fertilisation and starts the first cleavage division of the egg, which has no centriole. However, supplementation with these antioxidants did provide sperm from normo- and asthenozoospermic samples with similar levels of protection against H2O2-induced DNA damage and ROS production.
Hypotaurine and taurine ratios are lower in serum than in tubal and follicular fluids. Upon closer inspection, the researchers revealed that some of these resistant regions are associated with certain conditions, including diabetes, obesity and schizophrenia.
Glutathione could also function in the detoxification of peroxides through its interaction with sperm GPX, although the limited membrane permeability of GSH would reduce the effectiveness of this particular mechanism Baker et al.
Sperm collection After running of treatment period, each mouse was sacrificed by decapitation according to recommendation of the institutional ethical committee.
Alternatively, the neutral comet assay is technically simpler to use, is more cost efficient, and has a comparably higher sensitivity for detecting DNA damage.
Thus, hypotaurine in genital fluids may have protective functions for gametes and embryos. Although criticised as being an "imperfect tool" 4when performed under strict methodological guidelines and quality control, the basic semen analysis may provide useful information regarding male fertility potential.
Pyridaben is twice more potent than rotenone at inhibiting mitochondrial respiration. Antioxidants were made up in BWW and were present throughout the preparation procedure. These samples were subdivided and each control and antioxidant treatment group consisted of eight normozoospermic and seven asthenozoospermic samples.
In view of the non-Gaussian distribution of data, the non-parametric Wilcoxon matched pairs test was employed to determine the effects of glutathione and hypotaurine supplementation on sperm motility, DNA integrity and ROS production. A deviation of one or more semen parameters from the reference ranges can serve as an indication that a male factor may be implicated in the infertility problem encountered by the couple Thick smears of washed spermatozoa were prepared on pre-cleaned degreased slides and allowed to air-dry for 10 min.
To substantiate differences between the experimental conditions, the spectra were analyzed by Principal Component Analysis. Reduced glutathione GSH is present in many organs and tissues of the body and has many antioxidant properties Meister, This was a significantly greater reduction in intact DNA than that recorded for normozoospermic samples.
Two slides were prepared for each control and each test antioxidant to ensure that DNA damage could be induced at a later stage while still retaining an undamaged replicate. The Acidic Aniline Blue AAB stain specifically reacts with lysine residues in nuclear histones and reveals differences in the basic nuclear protein composition of the sperm.
It undergoes its secondary meiotic division, and the two haploid nuclei paternal and maternal fuse to form a zygote. At hours post HCG injection, female mice were scarified by cervical dislocation.
This means that any methylation that has occurred in these regions is not removed and can thus persist, potentially impacting future generations. Unlike other cells, spermatozoa are more vulnerable to DNA damage because they do not have the capacity for DNA repair.
The fertility potential in male mice was also evaluated. It has been indicated that, pyridaben miticide inhabitation of NADH: Moreover, there is debate regarding the relationship between the sperm DNA integrity and the clinical outcome.
Nevertheless, the presence of a low value per se may not preclude the possibility of the initiation of an in vivo pregnancy or, contrastingly, a "normal" spermiogram does not necessarily guarantee satisfactory fertilizing potential 2.
Sperm cells were removed from cauda epididymis and analyzed for chromatin integrity and DNA damage. During the recent years, the role of conventional semen analysis has been subjected to harsh criticism, characterized as "obsolete".
The pH was adjusted to 3. Hypotaurine is a preventative antioxidant that scavenges the hydroxyl radicals involved in the initiation of peroxidative damage. The 'tail' of the human sperm is predictably used to propel them through liquids, the 'head' contains small energy factories called mitochondria, in addition to the cell's DNA - packaged into twenty three chromosomes.
human sperm DNA damage may adversely affect reproductive outcomes and that spermatozoa of infertile men possess substantially more DNA damage than do spermatozoa of fertile men. samples are exposed to UVA, UVB, and UVC radiation, respectively for different periods of exposure.
Forensic techniques utili zed included DNA extraction from human male sperm cells through organic methods, genomic human DNA quantitation using Quantifiler®Y Human Male DNA Quantification Kit on ABI Real -Time PCR. In vitro study of the effect of various factors on DNA of Human sperms.
Dave Avani 1, Jain NK 1, Patel Himanshu 2, Patel Madhuri 2, Bhatt Vidisha 2, Patel Komal 2, Mallick Sarada 3, *Srivastava Pradeep 3. Abstract. Aim: Effect of various clinical compound and environmental conditions were studied for the in vitro fragmentation of the human.
Effect of glutathione and hypotaurine supplementation in vitro (10 mM) on H 2 O 2-induced DNA damage in human sperm Supplementation with glutathione and/or hypotaurine provided sperm with protection against H 2 O 2 -induced DNA damage.
View Avani Dave’s profile on LinkedIn, the world's largest professional community. In vitro study of the effect of various factors on DNA of Human sperms.
Education. Red River College Diploma, In vitro study of the effect of Tetracycline, Bisphenol A, Monosodium Glutamate and Temperature on DNA of human douglasishere.com: Research Assistant.Effect of various factors on dna of human sperms